Institute of Biomolecular Sciences Areas of Expertise

 

 

PERSON

EXPERTISE

ABLER

Cloning and vector construction, activity gels, genetic analysis of mutations, Bioinformatics,

ABLER

mapping and cloning specific mutations, DNA and RNA isolation/analysis, Plant growth and development

BRATINA

Use of 16S rRNA-based molecular tools for ecological studies/phylogenetic analyses

COOPER

Basic biochemistry and molecular biology

COOPER

Bioinformatics

GALBRAITH

Genetics

GALBRAITH

Molecular Biology (tfn, electrophoresis, cloning, PCR)

GALBRAITH

Western blotting

GALBRAITH

Co-immunoprecipitation

GRUNWALD

Protein purification techniques

GRUNWALD

Crosslinking techniques

GRUNWALD

SDS-PAGE and Westerns

GRUNWALD

Anaerobic techniques

GRUNWALD

Enzyme Assays

HOFFMAN

Lots of molecular/immunologic techniques:

HOFFMAN

Western, Far-Western, Northern, Sequencing, Primer Extension, Ribonuclease

HOFFMAN

Protection Assays, Immunoprecipitations, in vitro transcription, in vitro

HOFFMAN

translation, just about every type of plasmid manipulation known

HOFFMAN

Cell culture - transient transfection and protein expression in cell

HOFFMAN

culture, RNA extraction, sub-cellular fractionation via ultracentrifugation,

HOFFMAN

sucrose/glycerol gradients and cushions, immunofluorescence

HOFFMAN

Protein expression in E coli

HOWARD

Microscopy and image analysis of all kinds

HOWARD

TEM, SEM, fluorescence, immunochemistry, histochemistry, video, confocal, and image deconvolution

HOWARD

Protein biochemistry -- purification & characterization

HOWARD

Non-traditional protein activity assays (motor protein assays, etc.)

HOWARD

Cell fractionation

HOWARD

Cell culture--primary & long term

HOWARD

Chlamydomonas biology

HOWARD

Genetics

HOWARD

Protein labeling  (radioactivity & biochemical, e.g. biotinylation)

HOWARD

Kinase assays

HOWARD

Inhibitor/activator approaches

HOWARD

Handling, use, biology of invertebrates

HOWARD

Native gel electrophoresis

HOWARD

Live cell and tissue approaches to molecular questions

LOH

Spectroscopy

LOH

A little statistical mechanics/protein folding theory

LOH

Peptide synthesis

LOH

Protein dynamics (theory and NMR data acquisition)

MAHER

limited experience with northern blot, western blot, differential display

MAHER

Histochemistry (specifically muscle fiber typing)

MAHER

Handling, breeding, dosing, blood sampling, and surgery with rodent models

MAHER

Cell (cultured and freshly isolated), tissue, and animal physiology techniques

MAHER

Experience with IRB (human subjects) and IACUC (animal) protocols

MAHER

Design and completion of nutritional and exercise intervention studies with humans/animal models

MISKOWSKI

PCR, cloning, Southern and Northern blots

MISKOWSKI

Materials for (plasmids) and expertise with making/analyzing GFP reporter constructs

MISKOWSKI

Experience with performing/analyzing RNA-mediated interference (RNAi) experiments

MISKOWSKI

Performing/analyzing immunolocalization experiments

MISKOWSKI

Designing and conducting genetic screens, constructing strains, and strain maintenance

MISKOWSKI

Bioinformatics

MONTE

Protein-ligand interactions, organic synthesis and structure determination

ROTT

Cloning strategies

ROTT

DNA sequencing

ROTT

Bioinformatics

ROTT

PCR

ROTT

Hybridizations

SCHWAN

Genomic DNA isolation, RNA isolation, plasmid isolation, cloning, restriction endonuclease digestion,

SCHWAN

Northern blots, Southern blots, Western blots

SCHWAN

horizontal gel electrophoresis, manual sequencing, transposon mutagenesis

SCHWAN

conjugation, transduction, transformation, transfection,

SCHWAN

electroporation, gel mobility shifts, primer extension, DNAse footprinting,

SCHWAN

PCR, DD-PCR, RT-PCR, LDPCR, LDRT-PCR, signature-tagged mutagenesis,

SCHWAN

representational difference analysis, library construction (DNA and

SCHWAN

cDNA-based), tissue culturing of various cell lines, bacterial invasion

SCHWAN

adherence and invasion assays,

SCHWAN

immunofluorescence, immunoelectronimmunoprecipitation, ELISA and EIA, protein chromatography with microscopy

SCHWAN

DEAE ans sizing columns, radioactive proline assay

SCHWAN

intraperitoneal, intravenous and urinary tract administration of bacteria into animals, minor animal surgery

TAYLOR

Immunization of laboratory animals and production of antibodies, mammalian

TAYLOR

cell culture, monoclonal antibody production purification and

TAYLOR

characterization, assessment of immune cell function, and development of

TAYLOR

antibody-based assays to detect and measure immune system factors or

TAYLOR

exposure to infectious disease agents.  Commonly used techniques include

TAYLOR

flow cytometry, immunohistochemistry, ELISA, Western blot, protein

TAYLOR

purification, immunoprecipitation of membrane proteins, lymphocyte

TAYLOR

proliferation.

WEAVER

Protein crystallography, purification, engineering and enzyme kinetics

WEAVER

bio-informatics

WEAVER

SDS-PAGE, molecular weight determination via gel filtration, western blot analysis