Institute of Biomolecular Sciences Areas of Expertise |
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PERSON |
EXPERTISE |
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ABLER |
Cloning and vector construction, activity gels, genetic analysis of mutations, Bioinformatics, |
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ABLER |
mapping and cloning specific mutations, DNA and RNA isolation/analysis, Plant growth and development |
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BRATINA |
Use of 16S rRNA-based molecular tools for ecological studies/phylogenetic analyses |
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COOPER |
Basic biochemistry and molecular biology |
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COOPER |
Bioinformatics |
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GALBRAITH |
Genetics |
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GALBRAITH |
Molecular Biology (tfn, electrophoresis, cloning, PCR) |
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GALBRAITH |
Western blotting |
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GALBRAITH |
Co-immunoprecipitation |
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GRUNWALD |
Protein purification techniques |
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GRUNWALD |
Crosslinking techniques |
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GRUNWALD |
SDS-PAGE and Westerns |
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GRUNWALD |
Anaerobic techniques |
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GRUNWALD |
Enzyme Assays |
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HOFFMAN |
Lots of molecular/immunologic techniques: |
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HOFFMAN |
Western, Far-Western, Northern, Sequencing, Primer Extension, Ribonuclease |
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HOFFMAN |
Protection Assays, Immunoprecipitations, in vitro transcription, in vitro |
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HOFFMAN |
translation, just about every type of plasmid manipulation known |
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HOFFMAN |
Cell culture - transient transfection and protein expression in cell |
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HOFFMAN |
culture, RNA extraction, sub-cellular fractionation via ultracentrifugation, |
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HOFFMAN |
sucrose/glycerol gradients and cushions, immunofluorescence |
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HOFFMAN |
Protein expression in E coli |
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HOWARD |
Microscopy and image analysis of all kinds |
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HOWARD |
TEM, SEM, fluorescence, immunochemistry, histochemistry, video, confocal, and image deconvolution |
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HOWARD |
Protein biochemistry -- purification & characterization |
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HOWARD |
Non-traditional protein activity assays (motor protein assays, etc.) |
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HOWARD |
Cell fractionation |
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HOWARD |
Cell culture--primary & long term |
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HOWARD |
Chlamydomonas biology |
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HOWARD |
Genetics |
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HOWARD |
Protein labeling (radioactivity & biochemical, e.g. biotinylation) |
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HOWARD |
Kinase assays |
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HOWARD |
Inhibitor/activator approaches |
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HOWARD |
Handling, use, biology of invertebrates |
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HOWARD |
Native gel electrophoresis |
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HOWARD |
Live cell and tissue approaches to molecular questions |
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LOH |
Spectroscopy |
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LOH |
A little statistical mechanics/protein folding theory |
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LOH |
Peptide synthesis |
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LOH |
Protein dynamics (theory and NMR data acquisition) |
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MAHER |
limited experience with northern blot, western blot, differential display |
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MAHER |
Histochemistry (specifically muscle fiber typing) |
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MAHER |
Handling, breeding, dosing, blood sampling, and surgery with rodent models |
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MAHER |
Cell (cultured and freshly isolated), tissue, and animal physiology techniques |
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MAHER |
Experience with IRB (human subjects) and IACUC (animal) protocols |
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MAHER |
Design and completion of nutritional and exercise intervention studies with humans/animal models |
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MISKOWSKI |
PCR, cloning, Southern and Northern blots |
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MISKOWSKI |
Materials for (plasmids) and expertise with making/analyzing GFP reporter constructs |
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MISKOWSKI |
Experience with performing/analyzing RNA-mediated interference (RNAi) experiments |
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MISKOWSKI |
Performing/analyzing immunolocalization experiments |
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MISKOWSKI |
Designing and conducting genetic screens, constructing strains, and strain maintenance |
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MISKOWSKI |
Bioinformatics |
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MONTE |
Protein-ligand interactions, organic synthesis and structure determination |
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ROTT |
Cloning strategies |
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ROTT |
DNA sequencing |
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ROTT |
Bioinformatics |
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ROTT |
PCR |
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ROTT |
Hybridizations |
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SCHWAN |
Genomic DNA isolation, RNA isolation, plasmid isolation, cloning, restriction endonuclease digestion, |
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SCHWAN |
Northern blots, Southern blots, Western blots |
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SCHWAN |
horizontal gel electrophoresis, manual sequencing, transposon mutagenesis |
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SCHWAN |
conjugation, transduction, transformation, transfection, |
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SCHWAN |
electroporation, gel mobility shifts, primer extension, DNAse footprinting, |
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SCHWAN |
PCR, DD-PCR, RT-PCR, LDPCR, LDRT-PCR, signature-tagged mutagenesis, |
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SCHWAN |
representational difference analysis, library construction (DNA and |
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SCHWAN |
cDNA-based), tissue culturing of various cell lines, bacterial invasion |
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SCHWAN |
adherence and invasion assays, |
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SCHWAN |
immunofluorescence, immunoelectronimmunoprecipitation, ELISA and EIA, protein chromatography with microscopy |
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SCHWAN |
DEAE ans sizing columns, radioactive proline assay |
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SCHWAN |
intraperitoneal, intravenous and urinary tract administration of bacteria into animals, minor animal surgery |
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TAYLOR |
Immunization of laboratory animals and production of antibodies, mammalian |
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TAYLOR |
cell culture, monoclonal antibody production purification and |
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TAYLOR |
characterization, assessment of immune cell function, and development of |
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TAYLOR |
antibody-based assays to detect and measure immune system factors or |
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TAYLOR |
exposure to infectious disease agents. Commonly used techniques include |
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TAYLOR |
flow cytometry, immunohistochemistry, ELISA, Western blot, protein |
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TAYLOR |
purification, immunoprecipitation of membrane proteins, lymphocyte |
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TAYLOR |
proliferation. |
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WEAVER |
Protein crystallography, purification, engineering and enzyme kinetics |
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WEAVER |
bio-informatics |
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WEAVER |
SDS-PAGE, molecular weight determination via gel filtration, western blot analysis |