Separations

     

Fast Protein Liquid Chromatography (FPLC)

FLPC is used for the separation and analysis of protein mixtures. These systems operate at a slightly lower pressure than HPLC systems and are specialized for use with proteins.

GE AKTA UPC 10

  • Desktop controller
  • Frac-920 fraction controller
  • Various sample loops
  • BufferPrep kit

Contact: Todd Weaver

GE-AKTA-UPC-10

Gas Chromatography-Mass Spectrometry (GC-MS)

GC-MS is a sample separation technique relying on the volatility and size of particles. Samples are separated in a gaseous state and analyzed using their mass/charge ratio.

Agilent 7890A/5975C

  • Quadrupole mass spectrometer

Contact: Ressano Desouza-Machado

Agilent-7890A-5975C

High Performance Liquid Chromatography (HPLC)

Used to separate substances in solution using a variety of column choices and solvent compositions. UWL has a variety of different HPLC systems for different applications.

Agilent 1260 Infinity

  • Detection: variable wavelength UV-vis absorbance and fluorescence

Contact: Nadia Carmosini

Agilent-1260-Infinity

Agilent 1100

  • Detection: Variable wavelength UV-vis, multi-angle light scattering (Wyatt miniDAWN), and refractive index (Optilab)

Contact: Todd Weaver

Agilent-1100

HP 1100

 

  • Detection: UV-vis absorbance

 

Contact: Robert McGaff

 

Waters

  • Detection: light fluorescence and absorbance

Contact: Marc Rott

 

Ion Chromatography Unit

Liquid chromatography system used for quantitative and qualitative analysis of anions and cations in solution. This separation technique relies on the affinity of ions for a charged resin in the ion exchange column. 

Dionex ICS-90

  • Conductivity detector

Contact: Aric Opdahl

Dionex-ICS-90