Separations

    Fast Protein Liquid Chromatography (FPLC)

    FLPC is used for the separation and analysis of protein mixtures. These systems operate at a slightly lower pressure than HPLC systems and are specialized for use with proteins.

    GE AKTA UPC 10

    • Desktop controller
    • Frac-920 fraction controller
    • Various sample loops
    • BufferPrep kit

    Contact: Todd Weaver

    GE-AKTA-UPC-10

    Gas Chromatography-Mass Spectrometry (GC-MS)

    GC-MS is a sample separation technique relying on the volatility and size of particles. Samples are separated in a gaseous state and analyzed using their mass/charge ratio.

    Agilent 7890A/5975C

    • Quadrupole mass spectrometer

    Contact: Ressano Desouza-Machado

    Agilent-7890A-5975C

    High Performance Liquid Chromatography (HPLC)

    Used to separate substances in solution using a variety of column choices and solvent compositions. UWL has a variety of different HPLC systems for different applications.

    Agilent 1260 Infinity

    • Detection: variable wavelength UV-vis absorbance and fluorescence

    Contact: Nadia Carmosini

    Agilent-1260-Infinity

    Agilent 1100

    • Detection: Variable wavelength UV-vis, multi-angle light scattering (Wyatt miniDAWN), and refractive index (Optilab)

    Contact: Todd Weaver

    Agilent-1100

    HP 1100

     

    • Detection: UV-vis absorbance

     

    Contact: Robert McGaff

     

    Waters

    • Detection: light fluorescence and absorbance

    Contact: Marc Rott

     

    Ion Chromatography Unit

    Liquid chromatography system used for quantitative and qualitative analysis of anions and cations in solution. This separation technique relies on the affinity of ions for a charged resin in the ion exchange column. 

    Dionex ICS-90

    • Conductivity detector

    Contact: Aric Opdahl

    Dionex-ICS-90