College of Science & Health (CSH)
Spectroscopy
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Atomic Emission Spectrometer (AES)
The AES is an emission spectrometer used for qualitative and quantitative elemental analysis at ppb levels of liquid samples.
Agilent MP-AES
- Nitrogen based microwave plasma
- Monochromator and CCD detection
- Low ppb detection limits of environmental sensitive elements (e.g. arsenic, selenium, mercury)
Contact: Sarah Lantvit, Kristofer Rolfhus, Ressano Desouza-Machado, or Aric Opdahl
Circular Dichroism (CD) Spectrometer
Using circularly polarized light, CD is used to look at chiral molecules, particularly large biomolecules. This technique is useful in determining secondary structure.
Aviv 420
- Spectral range: 160-850 nm
- Air-cooled Xenon Lamp
- Thermostated cell holder with peltier device
Contact: Todd Weaver, Adrienne Loh, or Daniel Grilley
Double Beam UV-vis Spectrometer
Agilent Cary 5000
- Low=noise with a PbS photocell detector
- Spectral range of 175-3300 nm
- Resolution of <0.1 nm (UV and vis) and <0.2 nm (NIR)
- Max absorbance of 8
- 6 sample/6 reference temperature controlled sample holder (-10 C to 100 C)
- Solid sample transmission measurements
Contact: Sarah Lantvit or Aric Opdahl
Shimadzu UV-PC2101
- Spectral range of 190-900 nm
- Resolution ~0.1 nm
Contact: Aric Opdahl
Perkin-Elmer Lambda 2
- Spectral range of 190-1100 nm
- Configured for transmittance, absorbance, and reflectance measurements of thin films
Contact: Seth King
Fluorescence Spectrometer
Used to measure fluorescent output from liquid samples.
Shimadzu PC 5301
- Fluorescence and phosphorescence measurements
- Double scanning monochromators for selection of excitation and emission wavelengths
- Thermostatted sample cell
Contact: Aric Opdahl or Sarah Lantvit
Fourier Transform Infrared Spectrometer (FTIR)
Bruker FTIR instruments are versatile analytical tools equipped with options for characterization of a variety of samples in the mid-infrared region. Infrared spectrometry is a common technique is a common technique used to identify chemical functional groups.
Bruker Equinox 55 and Tensor 37
- Detector options: DTGS and liquid nitrogen cooled MCT for enhanced sensitivity
- Accessories for measuring gas, liquid, and solid samples
- Equipped with a specular reflection stage and ATR stage for surface analysis
Contact: Aric Opdahl or Keith Beyer
Laser Scattering Molecular Size and Zeta Potential
Used for measuring particle and molecule size. Can additionally be used to determine the zeta, or electrostatic, potential of the substance.
Malvern Zeta Sizer
- Sizer range of 1 nm to 10 µm
- Can additionally be used to determine the zeta, or electrostatic potential of the substance
- Surface zeta potential probe for studying adsorption energies
- Autotitration system
Contact: Sarah Lantvit, Todd Weaver, or Seth King
Malvern Mastersizer 2000
- Size range of 0.2 to 2000 µm
- Equipped with Hydro 2000G large volume automated wet dispersion accessory
Contact: Colin Belby or Sarah Lantvit
Microplate Reader
The microplate reader allows for the spectroscopic measurement of an entire well plate of samples. The sample size is also decreased, requiring only microliter amounts to obtain a reading. Thus, sample throughput is optimized. Plates are measured within the reader, away from any light interference.
BioTek Synergy 2
- Time-resolved fluorescence, AlphaScreen, luminescence, and UV-vis absorbance
- TAKE3 and automated injection modules available
- Gen5 reader control and data analysis software
- Utilizes a wide variety of plate types
- Recipe creation to add shaking, temperature regulation, and incubation steps
- Additional capabilities include well monitoring and kinetic measurements
Contact: Todd Weaver
SpectraMax 190
- UV-vis absorbance
- Spectral range is 190-850 nm
- Endpoint, kinetic, and scanning modes
- Temperature regulation from ambient to 45 C
- Automix feature to mix samples
Contact: Todd Weaver
Spectroscopic Ellipsometer
Ellipsometry measures the change in polarization of reflected or transmitted light. As such, it is primarily used to look at film thickness and optical constants.
Woollam Alpha-SE
- 65, 70, 75, and 90 degree angles of incidence
- Spectral range of 380-900 nm
- 500 µL liquid/flow cell (windows fixed at 70 degree incidence)
Contact: Seth King
Surface Plasmon Resonance Spectrometer (SPR)
SPR is a reflectivity measurement sensitive to the adsorption of molecules onto a thin metal sensor (typically gold). SPR sensors can be functionalized with a wide range of chemistries, and are generally used to study adsorption behavior, including biomolecular interactions. A primary advantage of this technique over fluorescence based analysis of biomolecules is that it does not require chemical labeling.
Various
- CCD detection
- Multiplex analysis enabled
- Wavelength and angle scanning
- Robust sample handling including degassing stages and temperature control from -10 C to +100 C
Contact: Aric Opdahl
UV-vis Spectrometer
Thermo NanoDrop 2000
- Spectral range of 190-840 nm
- Sample size of 0.5-2 µL
- Ideal for DNA, RNA, and protein
- Pre-configured for use with many common assays (i.e. DNA, Pierce 660, Bradford, BCA, etc.)
Contact: Todd Weaver
Ocean Optics DH 2000
- Modular spectroscopy system
- Spectral range of 215-2500 nm
Contact: Aric Opdahl
